| ! | Black & Gold Meet Records | 46.77 | Bryant | 3/29/2024 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Johnson & Wales (R.I.) |
A | Charles | Marinez | Plante | Preble | NT | |
| 3 | Rhode Island |
B | Best | Ball | London | Mor | 48.87 | |
| 4 | Bryant |
A | Braswell | Kane | Pineda | Haddad | 46.77 | Black & Gold Invitational |
| 5 | Rhode Island |
A | Santana | Federico | Colella | van Wyk | 46.87 | UCF Black & Gold Challenge |
| 6 | RIC |
A | Kamara | Hayes | Cary | Pezza | 49.44 | Alan Connie Shamrock Invitational |